It is the specific binding extrapolated to very high concentrations of radioligand, and so its value is almost always higher than any specific binding measured in your experiment. Modelīmax is the maximum specific binding in the same units as Y. Then use the Remove Baseline analysis to subtract column B from column A, creating a new results table with the specific binding.įrom the table of specific binding, click Analyze, choose nonlinear regression, choose the panel of Saturation Binding equations, and choose One site specific binding. If you have several experimental conditions, place the first into column A, the second into column B, etc.Īn alternative approach would be to enter total binding into column A, and nonspecific into column B. Enter radioligand concentration into X, and specific binding into Y.
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